Coding

Part:BBa_K5477022:Design

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)


POR - Cytochrome P450 oxidoreductase codon optimized for Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
    Illegal NgoMIV site found at 699
    Illegal NgoMIV site found at 818
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our system, POR plays a critical role in enabling the CYP3A4 and other cytochrome P450 enzymes to function effectively.


Source

Homo sapiens

References